反向互补序列计算器
支持 DNA/RNA 的反向、互补与反向互补计算，兼容 FASTA 格式输入。
Reverse, Complement, and Reverse Complement Tool
DNA/RNA Sequence Manipulation Guide
Our tool offers three essential functions for bioinformatics sequence analysis: Reverse, Complement, and Reverse Complement. These are fundamental operations for tasks like primer design, sequence alignment, and ORF finding.
- Reverse: This function simply reverses the order of your input DNA or RNA sequence. It is useful for preparing sequences for alignment or comparison.
- Complement: This generates the corresponding complementary strand of your DNA or RNA sequence. It replaces each base with its complement (A with T/U, G with C, and vice versa).
- Reverse Complement: This is a two-step process that first reverses the sequence and then generates the complement. The reverse complement is crucial for finding features on the opposite strand of a DNA molecule.
Important Considerations:
- Supported Formats: The tool accepts both raw DNA/RNA sequences and FASTA-formatted sequences. If a FASTA header is detected, it will be preserved in the output.
- Large Datasets: This tool supports converting hundreds or thousands of sequences. You can download the result as a file after conversion.
- Ambiguity Codes: We support IUPAC ambiguity codes. Two-nucleotide codes are converted as R↔Y and K↔M, while S and W remain unchanged. Three-nucleotide codes are handled as B↔V and D↔H.
- Case Preservation: The case of the input sequence is preserved in the output, allowing you to mark regions of interest.
- Client-Side Processing: All calculations are performed directly in your browser. Your data remains private and is never sent to our servers.
Nucleotide Ambiguity Codes
Ambiguity codes are used in bioinformatics to represent positions in a DNA or RNA sequence where the exact nucleotide may not be known. Our tool supports the full range of IUPAC ambiguity codes, ensuring accurate conversions for complex sequences. Below is a detailed table of these codes and their complements, as handled by our tool.
| Code | Nucleotides | Complement |
|---|---|---|
| A | A | T |
| G | G | C |
| C | C | G |
| T | T | A |
| U | U | A |
| R | A or G (purine) | Y |
| Y | C or T (pyrimidine) | R |
| S | C or G (strong) | S |
| W | A or T (weak) | W |
| K | G or T (keto) | M |
| M | A or C (amino) | K |
| B | C, G, or T (not A) | V |
| V | A, C, or G (not T) | B |
| D | A, G, or T (not C) | H |
| H | A, C, or T (not G) | D |
| - | gap | unchanged |
| any other | any or unknown | unchanged |
使用方法
- 粘贴或输入 DNA/RNA 序列（支持原始序列或 FASTA）。
- 选择需要的操作：反向、互补或反向互补。
- 复制或导出结果，用于引物设计、链方向核对或注释分析。
常见问题
反向互补序列通常用在什么场景？
常用于查看另一条链上的特征、引物设计，以及处理链特异的注释与分析。
支持模糊碱基（IUPAC）吗？
支持。可处理 IUPAC 模糊碱基（例如 R↔Y、K↔M、B↔V、D↔H），并保留大小写。