Plate Map Generator for 96-well and 384-well Plates

Design 96-well or 384-well plate layouts with condition colors, role shapes, controls, and copyable TSV output.

Generate a plate map

30
Filled wells
66
Unused wells
6
Control wells

96-well plate

1
2
3
4
5
6
7
8
9
10
11
12
A
C1
C1
C1
C2
C2
C2
C3
C3
C3
B
C5
C5
C5
C6
C6
C6
C7
C7
C7
C
D
E
F
G
H
NC
NC
NC

Legend

C1
C2
C3
C4
C5
C6
C7
C8
Positive control
Negative control
Empty
Why the encoding matters

A plate map is a quality-control record. Color separates biological or treatment conditions, while shape marks sample, positive control, and negative control roles so failed wells and control problems are easier to audit later.

Scientific plate map design rules

Keep row and column coordinates visible because raw instrument output, failed wells, and repeat runs are traced by well ID, not by sample name alone.

Group technical replicates in a consistent direction, but avoid hiding controls. This makes edge effects, evaporation, and pipetting drift easier to spot during review.

Separate positive and negative controls from experimental samples so contamination, reagent failure, and background signal can be checked before interpreting conditions.

How to use the output

Copy the TSV into an ELN, spreadsheet, or plate reader template.
Use row-wise filling for simple sample lists and column-wise filling for dose-response or replicate blocks.
Competitor analysis note

This plate map generator keeps the useful parts of research plate planning: annotated wells, visible legends, quick templates, and TSV output that can move into an ELN, spreadsheet, or plate reader setup.