Lab Calculators
A curated set of free, practical calculators for common wet-lab and sequence-analysis tasks. Each page includes clear formulas, step-by-step instructions, and copy-friendly preparation notes.
27
Calculators
4
Categories
$0
Free
24/7
Available
DNA Ligation Molar Ratio Calculator
Easy
Popular
Calculate insert-to-vector molar ratios, DNA amounts, and pipetting volumes for ligation reactions.
Free
dna ligation calculator
ligation calculator
Primer Resuspension & Dilution Calculator
Easy
Popular
Calculate buffer volumes to resuspend dried primers and prepare working dilutions from stock solutions.
Free
primer resuspension calculator
oligo reconstitution calculator
Solution Dilution Calculator
Easy
Popular
Calculate V1, V2, or C2 using C1V1 = C2V2. Includes dilution factor and preparation steps.
Free
solution dilution calculator
dilution
DNA Copy Number Calculator
Easy
Popular
Convert DNA mass (ng) to copies (and back) using fragment length (bp).
Free
copy number calculator
dna copies
qPCR Efficiency Calculator
Easy
Compute qPCR amplification efficiency, amplification factor, and Ct spacing from a standard curve slope.
Free
qpcr efficiency
standard curve slope
Lab Unit Converter
Easy
Convert common lab units for volume, mass, and concentration scales.
Free
unit converter
lab units
Plate Map Generator for 96-well and 384-well Plates
Easy
Generate 96-well or 384-well plate maps with condition colors, role shapes, controls, and TSV output.
Free
plate map generator
96-well plate map
Buffer Calculator
Easy
Popular
Calculate phosphate, Tris, citrate, acetate, PBS, TBS, TE, and TAE buffer recipes by pH, concentration, and volume.
Free
buffer calculator
phosphate buffer calculator
Codon Usage Frequency Calculator
Easy
Popular
Count codons from DNA/RNA/FASTA input and compare codon frequency, GC3, and rare codons against expression hosts.
Free
codon usage calculator
codon frequency calculator
Punnett Square Generator
Easy
Popular
Build monohybrid and dihybrid crosses with genotype ratios, phenotype probabilities, and gamete tables.
Free
punnett square generator
punnett square calculator
Cell Plating & Split Ratio Calculator
Easy
Popular
Calculate cell plating master mix volumes, seeding densities, hemocytometer concentrations, and culture passaging split ratios.
Free
cell plating calculator
cell seeding calculator
Tm Calculator (Primer Melting Temperature)
Easy
Popular
Calculate DNA and RNA primer melting temperature (Tm) and PCR annealing temperature (Ta) using nearest-neighbor thermodynamics and salt corrections.
Free
tm calculator
primer tm calculator
Molarity Calculator (Mass & Concentration)
Easy
Popular
Calculate mass of solid solute, molar concentration (M, mM, µM), and solution volume for lab buffer and chemical preparation.
Free
molarity calculator
molar concentration calculator
Serial Dilution Calculator
Easy
Popular
Calculate serial dilution factors, transfer and diluent volumes, final concentrations, and multi-well plate pipetting recipes.
Free
serial dilution calculator
dilution factor calculator
IC50 & EC50 Calculator (Dose-Response)
Easy
Popular
Fit dose-response assay curves with 4-parameter logistic (4PL) Hill equations, calculate IC50, EC50, Hill slope, and convert to Ki using Cheng-Prusoff.
Free
ic50 calculator
ec50 calculator
Enzyme Kinetics Calculator (Km & Vmax)
Easy
Popular
Fit Michaelis-Menten hyperbolic velocity and Lineweaver-Burk double reciprocal plots to calculate Km, Vmax, kcat turnover, and catalytic efficiency.
Free
enzyme kinetics calculator
michaelis menten calculator
OD600 to Cell Count & Biomass Calculator
Easy
Popular
Convert bacterial and yeast OD600 spectrophotometer absorbance to cell density, total count, and dry cell biomass with linearity QC.
Free
od600 calculator
bacterial cell count calculator
qPCR 2^-ΔΔCt Gene Expression Calculator
Easy
Popular
Calculate qPCR relative expression fold changes using the Livak 2^-ΔΔCt method with triplicate standard deviation error propagation.
Free
delta delta ct calculator
2^-ddct calculator
NanoDrop Nucleic Acid & Purity Calculator
Easy
Popular
Calculate dsDNA, RNA, and ssDNA concentration from A260, evaluate A260/A280 and A260/A230 purity ratios, and detect contaminants.
Free
nucleic acid calculator
nanodrop calculator
Cell Doubling Time & Growth Rate Calculator
Easy
Popular
Compute exponential doubling time (td), specific growth rate constant (µ), generations, and cumulative Population Doubling Level (PDL).
Free
cell doubling time calculator
specific growth rate calculator
Protein Concentration Calculator (BCA, Bradford & A280)
Easy
Popular
Calculate protein concentration by interpolating BCA or Bradford standard curves, or by direct UV A280 Beer-Lambert analysis.
Free
protein concentration calculator
bca assay calculator
Restriction Digest Master Mix Calculator
Easy
Popular
Calculate single and double restriction enzyme master mix recipes, DNA mass/volume, and check glycerol ≤ 5% to prevent star activity.
Free
restriction digest calculator
double digest calculator
Transfection Master Mix Calculator
Easy
Popular
Scale plasmid DNA, Lipofectamine 2000/3000, and PEI reagent volumes across multi-well plates and flasks with two-tube protocol recipes.
Free
transfection calculator
lipofectamine 3000 calculator
Hardy-Weinberg Equilibrium & Chi-Square Calculator
Easy
Popular
Calculate allele frequencies (p, q), expected genotype counts, Chi-square goodness-of-fit (df=1), and autosomal recessive carrier risks.
Free
hardy weinberg calculator
hardy weinberg equilibrium chi square
SDS-PAGE Gel Casting Recipe Calculator
Easy
Popular
Generate Laemmli resolving (6% to 18%) and stacking (4%) polyacrylamide gel recipes scaled for 0.75 mm, 1.0 mm, and 1.5 mm cassettes.
Free
sds page gel calculator
polyacrylamide gel recipe
Dialysis & Diafiltration Buffer Exchange Calculator
Easy
Popular
Calculate solute depletion across multiple dialysis buffer exchanges or centrifugal spin filter cycles with MWCO retention QC.
Free
dialysis buffer exchange calculator
desalting calculator
Protein Molecular Weight & pI Calculator
Easy
Popular
Calculate protein molecular weight (average & monoisotopic Da/kDa), extinction coefficient ε280, isoelectric point (pI), net charge vs pH titration curve, and Kyte-Doolittle hydropathy profile.
Free
protein molecular weight calculator
protein mw calculator
Choose by lab task
If you already know the experiment type, start from the specific calculator entry.
96-well Plate Map Generator
Use this for qPCR, ELISA, small dose-response assays, and standard 8×12 plate layouts.
384-well Plate Map Generator
Use this for high-throughput screens, miniaturized assays, and denser condition layouts.
Phosphate and Tris Buffer Calculator
Calculate phosphate, Tris-HCl, citrate, acetate, and Good’s buffer recipes by pH, pKa, concentration, and volume.
Quick Start
Pick a category, open a calculator, and copy the result into your lab notes.
1
Choose a category
Chemistry, Biology, or Web Lab—start from the tab that matches your task.
2
Enter known values
Use consistent units and fill in the fields you already know.
3
Copy & execute
Copy the preparation steps or tables and follow them at the bench.
Open Dilution Calculator