These are canonical complementary sequences, not predictions of the tRNAs used by a species. Initiation depends on context; this tool looks up triplets and does not identify open reading frames.
How to interpret your result
Codon and anticodon
A codon is a triplet on mRNA. A tRNA anticodon pairs with it in the opposite direction. For AUG, the paired sequence is 3′–UAC–5′; reading that same anticodon from 5′ to 3′ gives CAU.
Input strand and reading frame
Enter RNA or the DNA coding strand, always 5′→3′. DNA T is converted to RNA U. The first base starts the first triplet; the tool does not search for a start codon or choose a reading frame. A DNA template strand must first be converted to its coding-strand reverse complement.
Why real tRNAs may differ
This tool uses canonical A–U and G–C pairing. Wobble between codon position 3 and the 5′ end of the anticodon can allow a tRNA to read more than one codon. Actual recognition depends on the organism and modified bases; these results are not a catalogue of its tRNAs.
5′–N N ③–3′
│ │ ↕
3′–N N ①–5′
Top: codon position 3. Bottom: anticodon position 1 at its 5′ end. N means any base; the arrow marks the wobble position.
Stop and start signals
UAA, UAG and UGA are stop codons in the standard code. Release factors recognize them, so no ordinary tRNA anticodon is reported. AUG encodes methionine; whether it starts translation depends on its context. Alternative genetic codes and recoding are outside this tool’s scope.
