Nicotiana tabacum Codon Usage Table

See the most used codons, highest-fraction synonymous codons, and full codon usage frequencies for Nicotiana tabacum.

Use frequency to find the most common codons in this organism, and use fraction to compare synonymous codon preference within the same amino acid family.

Escherichia coli Saccharomyces cerevisiae Arabidopsis thaliana Caenorhabditis elegans Cricetulus griseus Drosophila melanogaster Homo sapiens Spodoptera frugiperda Mus musculus Nicotiana tabacum Pichia pastoris Sus scrofa Rattus norvegicus Streptomyces Zea mays

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These settings control the summary cards and the full codon usage table below.

Top Codons by Frequency

Codons ranked by frequency per 1,000 codons in the selected organism.

GAT

Amino acid: D

Fraction 0.68

Freq 36.7

GAA

Amino acid: E

Fraction 0.55

Freq 35.0

AAG

Amino acid: K

Fraction 0.51

Freq 33.9

AAA

Amino acid: K

Fraction 0.49

Freq 32.3

GCT

Amino acid: A

Fraction 0.44

Freq 32.0

GAG

Amino acid: E

Fraction 0.45

Freq 29.1

AAT

Amino acid: N

Fraction 0.60

Freq 27.4

ATT

Amino acid: I

Fraction 0.50

Freq 27.3

Top Codons by Fraction

Synonymous codons ranked by fraction. Stop codons and single-codon amino acids are excluded.

GAT

Amino acid: D

Fraction 0.68

Freq 36.7

CAT

Amino acid: H

Fraction 0.61

Freq 13.3

AAT

Amino acid: N

Fraction 0.60

Freq 27.4

TTT

Amino acid: F

Fraction 0.58

Freq 24.4

CAA

Amino acid: Q

Fraction 0.58

Freq 21.3

TAT

Amino acid: Y

Fraction 0.57

Freq 17.8

TGT

Amino acid: C

Fraction 0.57

Freq 9.9

GAA

Amino acid: E

Fraction 0.55

Freq 35.0

Preferred Codons by Amino Acid

One preferred codon per amino acid, ranked by frequency to surface practical defaults.

GAT

Amino acid: D

Fraction 0.68

Freq 36.7

GAA

Amino acid: E

Fraction 0.55

Freq 35.0

AAG

Amino acid: K

Fraction 0.51

Freq 33.9

GCT

Amino acid: A

Fraction 0.44

Freq 32.0

AAT

Amino acid: N

Fraction 0.60

Freq 27.4

ATT

Amino acid: I

Fraction 0.50

Freq 27.3

GTT

Amino acid: V

Fraction 0.41

Freq 27.0

ATG

Amino acid: M

Fraction 1.00

Freq 24.8

Nicotiana tabacum (Tobacco): (Genetic code: Standard)

Print-friendly 2-column table in genetic code order. Frequency is shown per 1,000 codons.

Triplet	Amino acid	Fraction	Frequency/Thousand	Number	Triplet	Amino acid	Fraction	Frequency/Thousand	Number
TTT TTC TTA TTG	F F L L	0.58 0.42 0.14 0.24	24.4 17.8 12.5 21.9	10,395 7,569 5,334 9,315	TCT TCC TCA TCG	S S S S	0.26 0.14 0.23 0.07	20.3 10.7 17.7 5.4	8,634 4,542 7,543 2,290
TAT TAC TAA TAG	Y Y * *	0.57 0.43 0.41 0.19	17.8 13.5 1.1 0.5	7,573 5,737 486 224	TGT TGC TGA TGG	C C * W	0.57 0.43 0.41 1.00	9.9 7.6 1.1 11.6	4,233 3,225 455 4,921
CTT CTC CTA CTG	L L L L	0.26 0.14 0.10 0.12	23.7 12.2 9.2 10.4	10,079 5,176 3,935 4,431	CCT CCC CCA CCG	P P P P	0.37 0.13 0.40 0.10	18.8 6.7 20.0 4.9	8,012 2,862 8,540 2,098
CAT CAC CAA CAG	H H Q Q	0.61 0.39 0.58 0.42	13.3 8.6 21.3 15.5	5,650 3,672 9,069 6,618	CGT CGC CGA CGG	R R R R	0.15 0.08 0.11 0.08	7.4 4.0 5.1 3.7	3,173 1,688 2,179 1,573
ATT ATC ATA ATG	I I I M	0.50 0.25 0.25 1.00	27.3 13.7 13.9 24.8	11,626 5,823 5,914 10,543	ACT ACC ACA ACG	T T T T	0.39 0.19 0.33 0.09	20.8 10.0 17.4 4.6	8,844 4,251 7,420 1,946
AAT AAC AAA AAG	N N K K	0.60 0.40 0.49 0.51	27.4 18.6 32.3 33.9	11,684 7,922 13,750 14,437	AGT AGC AGA AGG	S S R R	0.17 0.13 0.32 0.26	13.3 10.0 15.5 12.5	5,657 4,262 6,586 5,309
GTT GTC GTA GTG	V V V V	0.41 0.17 0.17 0.25	27.0 11.4 11.2 16.6	11,505 4,855 4,755 7,057	GCT GCC GCA GCG	A A A A	0.44 0.17 0.31 0.08	32.0 12.6 23.0 5.8	13,610 5,373 9,805 2,490
GAT GAC GAA GAG	D D E E	0.68 0.32 0.55 0.45	36.7 16.9 35.0 29.1	15,612 7,219 14,896 12,401	GGT GGC GGA GGG	G G G G	0.34 0.17 0.34 0.15	23.1 11.4 23.5 10.5	9,831 4,854 10,006 4,482

Showing 64 entries for Nicotiana tabacum (Tobacco).

Frequency is measured per 1,000 codons. Fraction compares codons that encode the same amino acid.

Questions this table can answer

Which codons are most common in Nicotiana tabacum?

Which synonymous codons are preferred in Nicotiana tabacum?

Which codons should be checked before expression design?

How to use this page

Use the summary cards to find the most used and highest-fraction codons quickly.

Switch sorting in the full table when you want to compare by frequency, fraction, or count.

Use the full organism list and search codons/amino acids on the Codon Usage Frequency Table.

FAQ

What are the most used codons in Nicotiana tabacum?

Use the "Most Used Codons" section to see which codons appear most often in Nicotiana tabacum. The ranking is based on frequency per 1,000 codons.

Which codons have the highest fraction in Nicotiana tabacum?

Use the "Highest Fraction Codons" section to compare synonymous codons for the same amino acid. Single-codon amino acids and stop codons are excluded so the ranking reflects codon preference rather than trivial 1.00 values.

What is the difference between codon frequency and codon fraction in Nicotiana tabacum?

Frequency measures how often a codon appears per 1,000 codons in the dataset. Fraction measures how strongly that codon is preferred relative to alternative codons encoding the same amino acid.

How should I use this Nicotiana tabacum codon usage table for optimization?

Start with the preferred-codon summaries to identify likely defaults, then review the full table below before making codon optimization or expression design decisions.